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電化學(xué)檢測系統(tǒng)
電化學(xué)檢測系統(tǒng)

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快速掃描循環(huán)伏安法(FSCV)電化學(xué)檢測系統(tǒng),用于兒茶酚胺(i.e., dopamine, norepinephrine, and serotonin)的測量??梢杂糜跍y量自由清醒活動(dòng)狀態(tài)下的動(dòng)物。該遙測系統(tǒng)適用于小鼠。

FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals.

HOW IT WORKS

Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V/s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.

l Voltage Span: -1.1 V to +1.3 V

l Range: 250 – 400 V/s

l Sweeps/second: 5 - 10

l Points/sweep: 1000

Analysis

Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.

The TETHERED FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host PC. The system comes with Pinnacle’s FREE 8500 software.

1. The FSCV interface box provides access to stimulus lines and transmits data to PAL-8500 software.

2. A low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.

3. Signals are amplified and filtered at the head of the animal using our headstage, which ensures the delivery of clean, artifact-free data.

4. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors. Headmounts are affixed to the skull with dental acrylic and act as a connection port for the headstage.

CARBON FIBER

Pinnacle’s FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 μm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag/AgCl reference electrode.

Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

CARBON FIBER SENSORS

Carbon fiber sensors are ordered by cannula type and whether the researcher needs to remove them from the cannula for post-calibration. CFS-F sensors are fixed in the cannula and cannot be removed for post-calibration。

FSCV PAL-8500 SOFTWARE PACKAGE

The FSCV system includes Pinnacle’s FREE PAL-8500 software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording, which allows monitoring of animal behavior simultaneously with biogenic amine release.

Additional features of this software include:

? Background Subtraction

? 3D Visualization

? User-Selectable Filters

? Heat Maps

? Animated Voltammograms

? Export to Third-Party Packages

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