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Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below
應(yīng)用范圍:
·系統(tǒng)設(shè)置校準(zhǔn)
·2D STED測(cè)量
·3D STED PSF 反卷積
·跟蹤及漂移校準(zhǔn)
優(yōu)勢(shì):
·大小: 直徑 = 23 nm
·亮度: 高亮,高密度
·高度均一: 超級(jí)小的尺寸及強(qiáng)度分布
·靈活性: 顏色修改或者增加化學(xué)成分
Bead R | Bead O | Bead G | Bead B | |
顏色 | 紅色 | 桔色 | 綠色 | 藍(lán)色 |
可用染料 | ATTO647N | ATTO 594 | ATTO 542 | Oregon Green 488 |
可替代染料 | Abberior STAR 635P | - | Cy3 or Alexa Fluor 555 | Alexa Fluor 488 or ATTO 490LS |
推薦激光源 | 630-650 nm | 565-610 nm | 515-540 nm | 480-505 nm |
尺寸 | 23 nm | 23 nm | 23 nm | 23 nm |
表面密度 | ≈ 1/μm2 | ≈ 1/μm2 | ≈ 1/μm2 | ≈ 1/μm2 |
熒光染料 | 顏色定義 | 激發(fā)光波長(zhǎng) / nm | 發(fā)射光波長(zhǎng) / nm |
ATTO 647N | R | 647 | 664 |
Abberior Star 635P | R | 635 | 651 |
ATTO 594 | O | 594 | 626 |
ATTO 542 | G | 542 | 562 |
Cy3 | G | 554 | 568 |
Alexa Fluor 555 | G | 555 | 580 |
Oregon Green 488 | B | 488 | 526 |
Alexa Fluor 488 | B | 488 | 525 |
ATTO 490LS | B | 490 | 658 |
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